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ADRA1D Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - ADRA1D Antibody (N-term) AW5131
    Western blot analysis of lysates from A549 cell line,rat brain tissue,LNCaP,PC-3 cell line (from left to right), using ADRA1D Antibody (N-term)(Cat. #AW5131). AW5131 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.
  • 4 - ADRA1D Antibody (N-term) AW5131
    Flow cytometric analysis of MCF-7 cells using ADRA1D Antibody (N-term)(green, Cat#AW5131) compared to an isotype control of rabbit IgG(blue). AW5131 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
  • 14 - ADRA1D Antibody (N-term) AW5131
    Immunohistochemical analysis of paraffin-embedded H. kidney section using ADRA1D Antibody (N-term)(Cat#AW5131). AW5131 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - ADRA1D Antibody (N-term) AW5131
    Immunohistochemical analysis of paraffin-embedded H. small intestine section using ADRA1D Antibody (N-term)(Cat#AW5131). AW5131 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 14 - ADRA1D Antibody (N-term) AW5131
    Immunohistochemical analysis of paraffin-embedded R. kidney section using ADRA1D Antibody (N-term)(Cat#AW5131). AW5131 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB
Primary Accession P25100
Reactivity Mouse, Rat, Human
Predicted Rabbit, Dog, Sheep, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 60463 Da
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 146
Antigen Region 1-30 aa
Other Names Alpha-1D adrenergic receptor, Alpha-1A adrenergic receptor, Alpha-1D adrenoreceptor, Alpha-1D adrenoceptor, Alpha-adrenergic receptor 1a, ADRA1D, ADRA1A
Dilution IHC-P~~1:100~500
FC~~1:25
WB~~1:1000
Target/Specificity This ADRA1D antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1-30amino acids from the N-terminal region of human ADRA1D.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsADRA1D Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ADRA1D
Synonyms ADRA1A
Function This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This alpha-adrenergic receptor mediates its effect through the influx of extracellular calcium.

REFERENCES

Bruno J.F.,et al.Biochem. Biophys. Res. Commun. 179:1485-1490(1991).
Forray C.,et al.Mol. Pharmacol. 45:703-708(1994).
Schwinn D.A.,et al.J. Pharmacol. Exp. Ther. 272:134-142(1995).
Weinberg D.H.,et al.Biochem. Biophys. Res. Commun. 201:1296-1304(1994).
Esbenshade T.A.,et al.Mol. Pharmacol. 47:977-985(1995).

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