Anti-ELP2 Antibody

Western blot analysis of ELP2 expression in LO2 (A), HEK293T (B), A549 (C) whole cell lysates. (Predicted band size: 92 kD; Observed band size: 100 kD)
Immunofluorescent analysis of ELP2 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q6IA86
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	92500 Da

Additional Information
Gene ID	55250
Other Names	STATIP1; Elongator complex protein 2; ELP2; SHINC-2; STAT3-interacting protein 1; StIP1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ELP2. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ELP2
Synonyms	STATIP1
Function	Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (PubMed:29332244). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (PubMed:29332244).
Cellular Location	Cytoplasm. Nucleus

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.