Anti-Mannose Binding Protein Antibody

Western blot analysis of Mannose Binding Protein expression in HepG2 (A), AML12 (B), PC12 (C), LO2 (D) whole cell lysates. (Predicted band size: 26 kD; Observed band size: 26 kD)
Immunofluorescent analysis of Mannose Binding Protein staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P11226
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	26144 Da

Additional Information
Gene ID	4153
Other Names	COLEC1; MBL; Mannose-binding protein C; MBP-C; Collectin-1; MBP1; Mannan-binding protein; Mannose-binding lectin
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Mannose Binding Protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MBL2 (HGNC:6922)
Function	Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:14515269, PubMed:22966085, PubMed:7634089, PubMed:9087411). Specifically recognizes and binds the mannose moiety of carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway (PubMed:22966085, PubMed:2573758, PubMed:6643429, PubMed:8082295, PubMed:9087411). Upon SARS coronavirus-2/SARS-CoV-2 infection, activates the complement lectin pathway which leads to the inhibition SARS-CoV-2 infection and a reduction of the induced inflammatory response (PubMed:35102342). May bind DNA (PubMed:15145932).
Cellular Location	Secreted. Cell surface. Note=Specifically binds mannose moiety of carbohydrates on the surface of pathogens.
Tissue Location	Plasma protein produced mainly in the liver.