Anti-PI3K p101 Antibody

Rabbit polyclonal antibody to PI3K p101

Western blot analysis of PI3K p101 expression in rat brain (A) whole cell lysates.
Immunohistochemical analysis of PI3K p101 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q8WYR1
Other Accession	Q5SW28
Reactivity	Human, Mouse, Rat, Pig, Chicken
Host	Rabbit
Clonality	Polyclonal
Calculated MW	97348 Da

Additional Information
Gene ID	23533
Other Names	Phosphoinositide 3-kinase regulatory subunit 5; PI3-kinase regulatory subunit 5; PI3-kinase p101 subunit; Phosphatidylinositol 45-bisphosphate 3-kinase regulatory subunit; PtdIns-3-kinase regulatory subunit; Protein FOAP-2; PtdIns-3-kinase p101; p101-PI3K
Target/Specificity	Recognizes endogenous levels of PI3K p101 protein.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/50 - 1/100) IHC~~WB (1/500 - 1/1000), IHC (1/50 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	PIK3R5
Function	Regulatory subunit of the PI3K gamma complex. Required for recruitment of the catalytic subunit to the plasma membrane via interaction with beta-gamma G protein dimers. Required for G protein- mediated activation of PIK3CG (By similarity).
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:O02696}. Cytoplasm {ECO:0000250\|UniProtKB:O02696}. Cell membrane {ECO:0000250\|UniProtKB:O02696}; Peripheral membrane protein {ECO:0000250\|UniProtKB:O02696}. Note=Predominantly localized in the nucleus in absence of PIK3CG/p120. Colocalizes with PIK3CG/p120 in the cytoplasm. Translocated to the plasma membrane in a beta-gamma G protein-dependent manner. {ECO:0000250\|UniProtKB:O02696}
Tissue Location	Ubiquitously expressed with high expression in fetal brain compared to adult brain. Abundant expression is observed in cerebellum, cerebral cortex, cerebral meninges, and vermis cerebelli

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.