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Anti-SYT11 Antibody

Western blot analysis of SYT11 expression in Hela (A), A549 (B), CT26 (C), PC12 (D) whole cell lysates. (Predicted band size: 48 kD; Observed band size: 48 kD)
Immunofluorescent analysis of SYT11 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9BT88
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	48297 Da

Additional Information
Gene ID	23208
Other Names	KIAA0080; Synaptotagmin-11; Synaptotagmin XI; SytXI
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human SYT11. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SYT11 (HGNC:19239)
Synonyms	KIAA0080
Function	Synaptotagmin family member involved in vesicular and membrane trafficking which does not bind Ca(2+). Inhibits clathrin- mediated and bulk endocytosis, functions to ensure precision in vesicle retrieval. Plays an important role in dopamine transmission by regulating endocytosis and the vesicle-recycling process. Essential component of a neuronal vesicular trafficking pathway that differs from the synaptic vesicle trafficking pathway but is crucial for development and synaptic plasticity. In macrophages and microglia, inhibits the conventional cytokine secretion, of at least IL6 and TNF, and phagocytosis. In astrocytes, regulates lysosome exocytosis, mechanism required for the repair of injured astrocyte cell membrane (By similarity). Required for the ATP13A2-mediated regulation of the autophagy-lysosome pathway (PubMed:27278822).
Cellular Location	Cytoplasmic vesicle membrane; Single-pass membrane protein. Perikaryon {ECO:0000250\|UniProtKB:Q9R0N3}. Golgi apparatus, trans-Golgi network membrane {ECO:0000250\|UniProtKB:Q9R0N3}; Single-pass membrane protein {ECO:0000250\|UniProtKB:Q9R0N3}. Recycling endosome membrane {ECO:0000250\|UniProtKB:Q9R0N3}; Single-pass membrane protein {ECO:0000250\|UniProtKB:Q9R0N3}. Lysosome membrane {ECO:0000250\|UniProtKB:Q9R0N3}; Single-pass membrane protein {ECO:0000250\|UniProtKB:Q9R0N3}. Cytoplasmic vesicle, phagosome {ECO:0000250\|UniProtKB:Q9R0N3}. Cell projection, axon. Cell projection, dendrite. Postsynaptic density {ECO:0000250\|UniProtKB:Q9R0N3}. Recycling endosome membrane {ECO:0000250\|UniProtKB:O08835}; Single-pass membrane protein {ECO:0000250\|UniProtKB:O08835}. Cytoplasmic vesicle, clathrin-coated vesicle membrane {ECO:0000250\|UniProtKB:O08835}; Single-pass membrane protein {ECO:0000250\|UniProtKB:O08835}. Perikaryon. Note=Localized in vesicles that travels in axonal and dendritic shafts in both anterograde and retrograde directions. In macrophages and microglia, recruited in phagosomes at early stages of phagocytosis (By similarity). Found in the core of the Lewy bodies in the brain of sporadic Parkinson disease patients (PubMed:12925569). {ECO:0000250\|UniProtKB:Q9R0N3, ECO:0000269\|PubMed:12925569}

Research Areas