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Anti-WEE1 (pS642) Antibody

Rabbit polyclonal antibody to WEE1 (pS642)

     
  • 1 - Anti-WEE1 (pS642) Antibody AP61084
    Western blot analysis of WEE1 (pS642) expression in mouse brain (A), rat brain (B) whole cell lysates.
Western blot analysis of WEE1 (pS642) expression in mouse brain (A), rat brain (B) whole cell lysates.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P30291
Other Accession P47810
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 71597 Da
Additional Information
Gene ID 7465
Other Names Wee1-like protein kinase; WEE1hu; Wee1A kinase
Target/Specificity Recognizes endogenous levels of WEE1 (pS642) protein.
Dilution WB~~WB (1/500 - 1/1000)
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name WEE1 {ECO:0000303|PubMed:8348613, ECO:0000312|HGNC:HGNC:12761}
Function Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15' (PubMed:15070733, PubMed:7743995, PubMed:8348613, PubMed:8428596). Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase (PubMed:7743995, PubMed:8348613, PubMed:8428596). Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur (PubMed:7743995, PubMed:8348613, PubMed:8428596). Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated (PubMed:7743995). A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation (PubMed:7743995).
Cellular Location Nucleus.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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