Anti-EPHA8 (Phospho-Y615) Antibody

Western blot analysis of EPHA8 (Phospho-Y615) expression in NIHC3T3 (A), H9C2 (B) whole cell lysates. (Predicted band size: 111 kD; Observed band size: 125 kD)
Immunofluorescent analysis of EPHA8 (Phospho-Y615) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P29322
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	111003 Da

Additional Information
Gene ID	2046
Other Names	EEK; HEK3; KIAA1459; Ephrin type-A receptor 8; EPH- and ELK-related kinase; EPH-like kinase 3; EK3; hEK3; Tyrosine-protein kinase receptor EEK
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y615 of human EPHA8 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	EPHA8
Synonyms	EEK, HEK3, KIAA1459
Function	Receptor tyrosine kinase which binds promiscuously GPI- anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The GPI-anchored ephrin-A EFNA2, EFNA3, and EFNA5 are able to activate EPHA8 through phosphorylation. With EFNA5 may regulate integrin-mediated cell adhesion and migration on fibronectin substrate but also neurite outgrowth. During development of the nervous system also plays a role in axon guidance. Downstream effectors of the EPHA8 signaling pathway include FYN which promotes cell adhesion upon activation by EPHA8 and the MAP kinases in the stimulation of neurite outgrowth (By similarity).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:O09127}; Single-pass type I membrane protein. Cell projection {ECO:0000250\|UniProtKB:O09127}. Early endosome membrane {ECO:0000250\|UniProtKB:O09127}. Note=Undergoes clathrin-mediated endocytosis upon EFNA5-binding and is targeted to early endosomes {ECO:0000250\|UniProtKB:O09127}

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.