Anti-BIK (Phospho-S35) Antibody

Western blot analysis of BIK (Phospho-S35) expression in mouse embryo (A), rat ovary (B), HEK293T-EGF (C), HEK293T (D) whole cell lysates. (Predicted band size: 18 kD; Observed band size: 18 kD)
Immunohistochemical analysis of BIK (Phospho-S35) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunohistochemical analysis of BIK (pS35) staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Direct ELISA antibody dose-response curve using Anti-BIK (Phospho-S35) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q13323
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	18016 Da

Additional Information
Gene ID	638
Other Names	NBK; Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S35 of human BIK protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IHC~~WB (1/500 - 1/1000), IHC (1/50 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	BIK {ECO:0000303\|PubMed:7478623, ECO:0000312\|HGNC:HGNC:1051}
Function	Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.
Cellular Location	Endomembrane system; Single-pass membrane protein. Mitochondrion membrane {ECO:0000250\|UniProtKB:O70337}; Single-pass membrane protein. Note=Around the nuclear envelope, and in cytoplasmic membranes.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.