Anti-Caspase 9 (pS144) Antibody

Rabbit polyclonal antibody to Caspase 9 (pS144)

Western blot analysis of Caspase 9 (pS144) expression in HEK293T (A), HepG2 (B), A375 (C) whole cell lysates.
Immunohistochemical analysis of Caspase 9 (pS144) staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P55211
Other Accession	Q8C3Q9
Reactivity	Human, Mouse, Monkey
Host	Rabbit
Clonality	Polyclonal
Calculated MW	46281 Da

Additional Information
Gene ID	842
Other Names	MCH6; Caspase-9; CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Caspase 9 (pS144). The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/50 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/50 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	CASP9
Synonyms	MCH6
Function	Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates effector caspases caspase-3 (CASP3) or caspase-7 (CASP7). Promotes DNA damage- induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC (PubMed:36758105, PubMed:36758106).
Tissue Location	Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.