Anti-ATP1A1 (Phospho-S23) Antibody

Western blot analysis of ATP1A1 (Phospho-S23) expression in HEK293T (A), MCF7 (B), DLD (C), SGC7901 (D) whole cell lysates. (Predicted band size: 112 kD; Observed band size: 100 kD)
Immunofluorescent analysis of ATP1A1 (Phospho-S23) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal

Additional Information
Other Names	ATP1A1; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1
Target/Specificity	KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S23 of human ATP1A1 protein. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt