Anti-MGST1 Antibody

Western blot analysis of MGST1 expression in rat liver (A), HCC827 (B), HepG2 (C) whole cell lysates. (Predicted band size: 17 kD; Observed band size: 18 kD)
Immunofluorescent analysis of MGST1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P10620
Reactivity	Human, Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	17599 Da

Additional Information
Gene ID	4257
Other Names	GST12; MGST; Microsomal glutathione S-transferase 1; Microsomal GST-1; Microsomal GST-I
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human MGST1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	MGST1
Synonyms	GST12, MGST
Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Cellular Location	Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:P08011}; Multi-pass membrane protein. Mitochondrion outer membrane {ECO:0000250\|UniProtKB:P08011}
Tissue Location	Highly expressed in liver.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.