癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GREMLIN Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 文献引用 : 10
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | O70326 |
| Other Accession | O35793, O60565 |
| Reactivity | Human, Mouse |
| Predicted | Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Gene ID | 23892 |
|---|---|
| Other Names | Gremlin-1, Cysteine knot superfamily 1, BMP antagonist 1, Down-regulated in Mos-transformed cells protein, Grem1, Cktsf1b1, Drm |
| Target/Specificity | This GREMLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the C-terminal region of human GREMLIN. |
| Dilution | WB~~1:2000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GREMLIN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Grem1 |
|---|---|
| Synonyms | Cktsf1b1, Drm |
| Function | Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop (PubMed:12808456, PubMed:15201225). Down-regulates the BMP4 signaling in a dose- dependent manner (PubMed:15133038). Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro) (By similarity). Acts as inhibitor of monocyte chemotaxis (By similarity). |
| Cellular Location | Secreted. |
| Tissue Location | Highly expressed in spleen and to a lesser extent in lung, skeletal muscle and kidney. Expressed only in non- transformed cells or primary fibroblasts in culture but not in established transformed or tumor derived cell lines. Broadly expressed in limb bud mesenchyme but restricted to the distal limb bud mesenchyme and concentrated posteriorly. Expressed in ovary especially in granulosa cells of follicles of type 4 |
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Author : Lavoz C1,Poveda J2,Márquez-Expósito L3,Rayego-Mateos S2,Rodrigues-Diez RR4,Ortiz A5,Egido J3,Mezzano S1,Ruiz-Ortega M6. Clin Sci (Lond). 2018 May 2. pii: CS20171553. doi: 10.1042/CS20171553. [Epub ahead of print] |
29720422 |
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Author : Staloch D1, Gao X, Liu K, Xu M, Feng X, Aronson JF, Falzon M, Greeley GH, Rastellini C, Chao C, Hellmich MR, Cao Y, Ko TC. J Mol Med (Berl). 2015 Jul 5. [Epub ahead of print] |
26141517 |
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Author : Droguett A1, Krall P1, Burgos ME1, Valderrama G1, Carpio D2, Ardiles L1, Rodriguez-Diez R3, Kerr B4, Walz K4, Ruiz-Ortega M3, Egido J3 PLoS One. 2014 Jul 18;9(7):e101879. doi: 10.1371/journal.pone.0101879. eCollection 2014. |
25036148 |
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Author : Ahn EH, Mercado GE, Laé M, Ladanyi M. Oncol Rep. 2013 Aug;30(2):968-78. doi: 10.3892/or.2013.2513. Epub 2013 Jun 3. |
23733015 |
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Author : Gelse K, Ekici AB, Cipa F, Swoboda B, Carl HD, Olk A, Hennig FF, Klinger P. Osteoarthritis Cartilage. 2012 Feb;20(2):162-71. doi: 10.1016/j.joca.2011.12.004. Epub 2011 Dec 13. |
22209871 |
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Author : Jara A, Chacón C, Burgos ME, Droguett A, Valdivieso A, Ortiz M, Troncoso P, Mezzano S. Nephrol Dial Transplant. 2009 Apr;24(4):1121-9. doi: 10.1093/ndt/gfn611. Epub 2008 Nov 21. |
19028757 |
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Author : Carvajal G, Droguett A, Burgos ME, Aros C, Ardiles L, Flores C, Carpio D, Ruiz-Ortega M, Egido J, Mezzano S. Transplant Proc. 2008 Apr;40(3):734-9. doi: 10.1016/j.transproceed.2008.02.064. |
18455002 |
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Author : Mezzano S, Droguett A, Burgos ME, Aros C, Ardiles L, Flores C, Carpio D, Carvajal G, Ruiz-Ortega M, Egido J. Nephrol Dial Transplant. 2007 Jul;22(7):1882-90. Epub 2007 Apr 1. |
17403698 |
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Author : Wordinger RJ, Fleenor DL, Hellberg PE, Pang IH, Tovar TO, Zode GS, Fuller JA, Clark AF. Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1191-200. |
17325163 |
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Author : Frank NY, Kho AT, Schatton T, Murphy GF, Molloy MJ, Zhan Q, Ramoni MF, Frank MH, Kohane IS, Gussoni E. J Cell Biol. 2006 Oct 9;175(1):99-110. Epub 2006 Oct 2. |
17015616 |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
GREMLIN is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth.
REFERENCES
Chen, B., et al., J. Immunol. 173(10):5914-5917 (2004). Chen, B., et al., Biochem. Biophys. Res. Commun. 295(5):1135-1141 (2002). McMahon, R., et al., J. Biol. Chem. 275(14):9901-9904 (2000). Murphy, M., et al., J. Biol. Chem. 274(9):5830-5834 (1999). Hsu, D.R., et al., Mol. Cell 1(5):673-683 (1998).
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