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>   首页   >   产品   >   一抗   >   信号转导   >   GREMLIN Antibody (C-term)   

GREMLIN Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - GREMLIN Antibody (C-term) AP6133a
    Immunohistochemical analysis of paraffin-embedded mouse lung section using GREMLIN Antibody (C-term) (Cat#AP6133a). AP6133a was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 14 - GREMLIN Antibody (C-term) AP6133a
    Immunohistochemical analysis of paraffin-embedded mouse spleen section using GREMLIN Antibody (C-term) (Cat#AP6133a). AP6133a was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 14 - GREMLIN Antibody (C-term) AP6133a
    Immunohistochemical analysis of paraffin-embedded human spleen section using GREMLIN Antibody (C-term) (Cat#AP6133a). AP6133a was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 14 - GREMLIN Antibody (C-term) AP6133a
    Immunohistochemical analysis of paraffin-embedded mouse kidney section using GREMLIN Antibody (C-term) (Cat#AP6133a). AP6133a was diluted at 1:200 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
  • 1 - GREMLIN Antibody (C-term) AP6133a
    Anti-GREMLIN Antibody (C-term) at 1:2000 dilution + Mouse skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession O70326
Other Accession O35793, O60565
Reactivity Human, Rat, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 20710 Da
Antigen Region 147-175 aa
Additional Information
Gene ID 23892
Other Names Gremlin-1, Cysteine knot superfamily 1, BMP antagonist 1, Down-regulated in Mos-transformed cells protein, Grem1, Cktsf1b1, Drm
Target/Specificity This GREMLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the C-terminal region of mouse GREMLIN.
Dilution IHC-P~~1~200
WB~~1:2000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGREMLIN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Grem1
Synonyms Cktsf1b1, Drm
Function Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop (PubMed:12808456, PubMed:15201225). Down-regulates the BMP4 signaling in a dose-dependent manner (PubMed:15133038). Antagonist of BMP2; inhibits BMP2-mediated differentiation of osteoblasts (in vitro) (By similarity). Acts as inhibitor of monocyte chemotaxis (By similarity).
Cellular Location Secreted.
Tissue Location Highly expressed in spleen and to a lesser extent in lung, skeletal muscle and kidney. Expressed only in non-transformed cells or primary fibroblasts in culture but not in established transformed or tumor derived cell lines. Broadly expressed in limb bud mesenchyme but restricted to the distal limb bud mesenchyme and concentrated posteriorly. Expressed in ovary especially in granulosa cells of follicles of type 4.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

GREMLIN is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth.

REFERENCES

Chen, B., et al., J. Immunol. 173(10):5914-5917 (2004). Chen, B., et al., Biochem. Biophys. Res. Commun. 295(5):1135-1141 (2002). McMahon, R., et al., J. Biol. Chem. 275(14):9901-9904 (2000). Murphy, M., et al., J. Biol. Chem. 274(9):5830-5834 (1999). Hsu, D.R., et al., Mol. Cell 1(5):673-683 (1998).

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