Anti-CD103 Antibody

Western blot analysis of CD103 expression in PMVEC (A), SP20 (B), SGC7901 (C), A549 (D) whole cell lysates. (Predicted band size: 130 kD; Observed band size: 150 kD)
Immunofluorescent analysis of CD103 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	P38570
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	130159 Da

Additional Information
Gene ID	3682
Other Names	Integrin alpha-E; HML-1 antigen; Integrin alpha-IEL; Mucosal lymphocyte 1 antigen; CD103
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CD103. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ITGAE
Function	Integrin ITGAE/ITGB7 (alpha-E/beta-7) is a receptor for E- cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
Cellular Location	Membrane; Single-pass type I membrane protein.
Tissue Location	Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.