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Anti-Histone H4 (MonoMethyl-K5) Antibody

Rabbit polyclonal antibody to Histone H4 (MonoMethyl-K5)

Western blot analysis of Histone H4 (MonoMethyl-K5) expression in Hela (A) whole cell lysates.
Immunohistochemical analysis of Histone H4 (MonoMethyl-K5) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Direct ELISA antibody dose-response curve using Anti-Histone H4 (MonoMethyl-K5) Antibody. Antigen (methyl-peptide and non-methyl-peptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P62805
Other Accession	P62806
Reactivity	Human, Mouse, Rat, Pig, Chicken, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	11367 Da

Additional Information
Gene ID	121504;554313;8294;8359;8360;8361;8362;8363;8364;8365;8366;8367;8368;8370
Other Names	H4/A; H4FA; H4/I; H4FI; H4/G; H4FG; H4/B; H4FB; H4/J; H4FJ; H4/C; H4FC; H4/H; H4FH; H4/M; H4FM; H4/E; H4FE; H4/D; H4FD; H4/K; H4FK; H4/N; H4F2; H4FN; HIST2H4; H4/O; H4FO; Histone H4
Target/Specificity	Recognizes endogenous levels of Histone H4 with a site at MonoMethyl-K5 protein.
Dilution	WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	H4C1
Synonyms	H4/A, H4FA, HIST1H4A
Function	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Cellular Location	Nucleus {ECO:0000250\|UniProtKB:P62806}. Chromosome. Note=Localized to the nucleus when acetylated in step 11 spermatids. {ECO:0000250\|UniProtKB:P62806}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Histone H4 with a site at MonoMethyl-K5. The exact sequence is proprietary.

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¥ 1,500.00

Cat# AP61436

Size:

50ul

100 µl

Price:

¥ 1,500.00

¥ 2,600.00

Quantity:

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Availability: 3-5 days

询价

Anti-Histone H4 (MonoMethyl-K5) Antibody

Rabbit polyclonal antibody to Histone H4 (MonoMethyl-K5)

BACKGROUND

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