Anti-Adenylate Kinase 1 Antibody

Rabbit polyclonal antibody to Adenylate Kinase 1

Western blot analysis of Adenylate Kinase 1 expression in A549 (A), A375 (B) whole cell lysates.
Immunohistochemical analysis of Adenylate Kinase 1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Adenylate Kinase 1 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF
Primary Accession	P00568
Other Accession	Q9R0Y5
Reactivity	Human, Mouse, Rat, Monkey, Pig, Chicken, Bovine, Dog, SARS
Host	Rabbit
Clonality	Polyclonal
Calculated MW	21635 Da

Additional Information
Gene ID	203
Other Names	Adenylate kinase isoenzyme 1; AK 1; ATP-AMP transphosphorylase 1; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; Myokinase
Target/Specificity	Recognizes endogenous levels of Adenylate Kinase 1 protein.
Dilution	WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) IHC~~1:100~500 IF~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	AK1 {ECO:0000255\|HAMAP-Rule:MF_03171, ECO:0000312\|HGNC:HGNC:361}
Function	Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism (By similarity) (PubMed:21080915, PubMed:23416111, PubMed:2542324). Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP (By similarity).
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:P05081}.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.