Anti-KCHIP1 Antibody

Rabbit polyclonal antibody to KCHIP1

Western blot analysis of KCHIP1 expression in CT26 (A), C6 (B), MCF7 (C), HEK293T (D), U87MG (E) whole cell lysates.
Immunohistochemical analysis of KCHIP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of KCHIP1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, IF
Primary Accession	Q9NZI2
Other Accession	Q9JJ57
Reactivity	Human, Mouse, Rat, Pig, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	26817 Da

Additional Information
Gene ID	30820
Other Names	KCHIP1; VABP; Kv channel-interacting protein 1; KChIP1; A-type potassium channel modulatory protein 1; Potassium channel-interacting protein 1; Vesicle APC-binding protein
Target/Specificity	Recognizes endogenous levels of KCHIP1 protein.
Dilution	WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) IHC~~1:100~500 IF~~WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	KCNIP1 (HGNC:15521)
Function	Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels (PubMed:10676964, PubMed:11423117, PubMed:17187064, PubMed:34552243, PubMed:34997220). Regulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed:10676964, PubMed:11423117, PubMed:17187064, PubMed:34552243, PubMed:34997220). In vitro, modulates KCND1/Kv4.1 and KCND2/Kv4.2 currents (PubMed:34552243). Increases the presence of KCND2 at the cell surface (PubMed:12829703).
Cellular Location	Cell membrane; Peripheral membrane protein. Cytoplasm. Cell projection, dendrite {ECO:0000250\|UniProtKB:Q8R426}
Tissue Location	Isoform 1 and isoform 2 are expressed in brain and kidney. Isoform 1 is also expressed in liver, pancreas, skeletal muscle, small intestine and testis. Isoform 2 is also expressed in lung, pancreas, leukocytes, prostate and thymus