Anti-Swiprosin-2 Antibody

Mouse monoclonal antibody to Swiprosin-2

Western blot analysis of Swiprosin-2 expression in mouse spleen (A), rat spleen (B) whole cell lysates.
Immunofluorescent analysis of Swiprosin-2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9BUP0
Other Accession	Q9D4J1
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	26928 Da

Additional Information
Gene ID	80303
Other Names	SWS2; EF-hand domain-containing protein D1; EF-hand domain-containing protein 1; Swiprosin-2
Target/Specificity	Recognizes endogenous levels of Swiprosin-2 protein.
Dilution	WB~~WB (1/1000 - 1/2000), IF/IC (1/100 - 1/200) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	EFHD1
Synonyms	SWS2
Function	Acts as a calcium sensor for mitochondrial flash (mitoflash) activation, an event characterized by stochastic bursts of superoxide production (PubMed:26975899). May play a role in neuronal differentiation (By similarity).
Cellular Location	Mitochondrion inner membrane {ECO:0000250\|UniProtKB:Q9D4J1}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.