Anti-TPP2 Antibody

Western blot analysis of TPP2 expression in Jurkat (A), SW620 (B), mouse brain (C), mouse spleen (D) whole cell lysates. (Predicted band size: 138 kD; Observed band size: 138 kD)
Immunohistochemical analysis of TPP2 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P29144
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	138350 Da

Additional Information
Gene ID	7174
Other Names	Tripeptidyl-peptidase 2; TPP-2; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase II; TPP-II
Target/Specificity	Recombinant fusion protein of human TPP2
Dilution	WB~~WB (1/500 - 1/1000) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TPP2
Function	Cytosolic tripeptidyl-peptidase that releases N-terminal tripeptides from polypeptides and is a component of the proteolytic cascade acting downstream of the 26S proteasome in the ubiquitin- proteasome pathway (PubMed:25525876, PubMed:30533531). It plays an important role in intracellular amino acid homeostasis (PubMed:25525876). Stimulates adipogenesis (By similarity).
Cellular Location	Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to gamma-irradiation

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.