癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
MAGEA3 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 文献引用 : 4
- 实验流程
- 背景知识
Application 
| WB, FC, IHC-P, E |
|---|---|
| Primary Accession | P43357 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 34747 Da |
| Antigen Region | 283-314 aa |
| Gene ID | 4102 |
|---|---|
| Other Names | Melanoma-associated antigen 3, Antigen MZ2-D, Cancer/testis antigen 13, CT13, MAGE-3 antigen, MAGEA3, MAGE3 |
| Target/Specificity | This MAGEA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-314 amino acids from the C-terminal region of human MAGEA3. |
| Dilution | WB~~1:2000 FC~~1:10~50 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | MAGEA3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | MAGEA3 {ECO:0000303|PubMed:29779948, ECO:0000312|HGNC:HGNC:6801} |
|---|---|
| Function | Activator of ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases that acts as a repressor of autophagy (PubMed:20864041, PubMed:31267705). May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex (PubMed:17942928, PubMed:20864041). May play a role in embryonal development and tumor transformation or aspects of tumor progression (PubMed:17942928, PubMed:20864041). In vitro promotes cell viability in melanoma cell lines (PubMed:17942928). Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes (PubMed:8113684). |
| Tissue Location | Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
MAGEA3 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.
REFERENCES
Guo, J., et al., Exp. Mol. Pathol. 74(2):140-147 (2003). Consogno, G., et al., Blood 101(3):1038-1044 (2003). Rogner, U.C., et al., Genomics 29(3):725-731 (1995). Gaugler, B., et al., J. Exp. Med. 179(3):921-930 (1994). van der Bruggen, P., et al., Science 254(5038):1643-1647 (1991).
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