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MMP9 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

All lanes : Anti-MMP9 Antibody (L659) at 1:2000 dilution Lane 1: NCI-H1299 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with MMP9 antibody (C-term) (Cat.#AP6214a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP9 antibody (C-term) (Cat.#AP6214a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

产品详情
文献引用 : 10
实验流程
背景知识

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, WB, E
Primary Accession	P14780
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig

Additional info
Gene ID	4318
Other Names	Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, MMP9, CLG4B
Target/Specificity	This MMP9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 644-673 amino acids from the C-terminal region of human MMP9.
Dilution	WB~~1:2000 IHC-P~~1:50~100
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	MMP9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	MMP9
Synonyms	CLG4B
Function	May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-\|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Cellular Location	Secreted, extracellular space, extracellular matrix
Tissue Location	Detected in neutrophils (at protein level) (PubMed:7683678). Produced by normal alveolar macrophages and granulocytes.

Research Areas

Effects of secreted frizzled-related protein 1 on proliferation, migration, invasion, and apoptosis of colorectal cancer cells.
Author : Wang Z#1,Li R#1,He Y1,Huang S1. Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.	29610564

Association of MMP-2 and MMP-9 expression with recurrences in primary spontaneous pneumothorax.
Author : Huang YF1,Chiu WC2,Chou SH2,Su YH3,Chen YW1,Chai CY4,Huang CJ5,Huang MY5,Yuan SF6,Lee YC7. Kaohsiung J Med Sci. 2017 Jan;33(1):17-23. doi: 10.1016/j.kjms.2016.10.007. Epub 2016 Nov 12.	28088269

Nkx2-5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E-Deficient Mice.
Author : Du M1,2,Wang X1,2,Tan X1,2,Li X1,2,Huang D1,2,Huang K1,2,Yang L1,2,Zhang F1,2,Wang Y1,2,Huang D1,2,Huang K3,2. J Am Heart Assoc. 2016 Dec 19;5(12). pii: e004440.	27993833

EFEMP1 inhibits migration of hepatocellular carcinoma by regulating MMP2 and MMP9 via ERK1/2 activity.
Author : Dou CY¹, Cao CJ², Wang Z², Zhang RH³, Huang LL³, Lian JY², Xie WL², Wang LT². Oncol Rep. 2016 Apr 5. doi: 10.3892/or.2016.4733. [Epub ahead of print]	27108677

Ethanol-Induced TLR4/NLRP3 Neuroinflammatory Response in Microglial Cells Promotes Leukocyte Infiltration Across the BBB.
Author : Alfonso-Loeches S¹, Ureña-Peralta J¹, Morillo-Bargues MJ¹, Gómez-Pinedo U², Guerri C³. Neurochem Res. 2015 Nov 11. [Epub ahead of print]	26555554

Down-regulation of TCF21 by hypermethylation induces cell proliferation, migration and invasion in colorectal cancer.
Author : Dai Y¹, Duan H², Duan C³, Zhou R¹, He Y¹, Tu Q¹, Shen L⁴. Biochem Biophys Res Commun. 2015 Oct 1. pii: S0006-291X(15)30626-4. doi: 10.1016/j.bbrc.2015.09.109. [Epub ahead of print]	26435499

Calreticulin promotes migration and invasion of esophageal cancer cells by upregulating neuropilin-1 expression via STAT5A.
Author : Shi F¹, Shang L¹, Pan BQ¹, Wang XM¹, Jiang YY¹, Hao JJ¹, Zhang Y¹, Cai Y¹, Xu X¹, Zhan QM¹, Wang MR². Clin Cancer Res. 2014 Dec 1;20(23):6153-62. doi: 10.1158/1078-0432.CCR-14-0583. Epub 2014 Sep 17.	25231404

High expression of Dickkopf-related protein 1 is related to lymphatic metastasis and indicates poor prognosis in intrahepatic cholangiocarcinoma patients after surgery.
Author : Shi RY, Yang XR, Shen QJ, Yang LX, Xu Y, Qiu SJ, Sun YF, Zhang X, Wang Z, Zhu K, Qin WX, Tang ZY, Fan J, Zhou J. Cancer. 2013 Mar 1;119(5):993-1003. doi: 10.1002/cncr.27788. Epub 2012 Nov 6.	23132676

A lipoxin A4 analog ameliorates blood-brain barrier dysfunction and reduces MMP-9 expression in a rat model of focal cerebral ischemia-reperfusion injury.
Author : Wu Y, Wang YP, Guo P, Ye XH, Wang J, Yuan SY, Yao SL, Shang Y. J Mol Neurosci. 2012 Mar;46(3):483-91. doi: 10.1007/s12031-011-9620-5. Epub 2011 Aug 16.	21845429

OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases.
Author : Chen KC¹, Wang YS, Hu CY, Chang WC, Liao YC, Dai CY, Juo SH. FASEB J. 2011 May;25(5):1718-28. doi: 10.1096/fj.10-174904. Epub 2011 Jan 25.	21266537

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

REFERENCES

Tutton, M.G., et al., Int. J. Cancer 107(4):541-550 (2003). Behrens, P., et al., Int. J. Cancer 107(2):183-188 (2003). Van den Steen, P.E., et al., Biochem. Biophys. Res. Commun. 310(3):889-896 (2003). Zhao, Z., et al., J. Infect. Dis. 188(8):1098-1104 (2003). Matsuyama, A., et al., Circulation 108(12):1469-1473 (2003).

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Discontinued

Cat# AP6214a

Size:

50 µl

100 µl

200 µl

400 µl

Price:

¥ 1,100.00

¥ 1,800.00

¥ 2,600.00

¥ 4,000.00

替代产品:

MMP9 Antibody, MMP-9 Polyclonal Antibody, MMP-9 Polyclonal Antibody,

MMP9 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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