- 文献引用 : 3
|Application ||IHC-P, WB, E|
|Calculated MW||115483 Da|
|Other Names||Zinc finger MIZ domain-containing protein 1, PIAS-like protein Zimp10, Retinoic acid-induced protein 17, ZMIZ1, KIAA1224, RAI17, ZIMP10|
|Target/Specificity||This RAI17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1038-1067 amino acids from the C-terminal region of human RAI17.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||RAI17 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||KIAA1224, RAI17, ZIMP10|
|Function||Acts as transcriptional coactivator. Increases ligand- dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation (PubMed:14609956, PubMed:26522984). Involved in transcriptional activation of a subset of NOTCH1 target genes including MYC. Involved in thymocyte and T cell development (By similarity).|
|Cellular Location||Nucleus speckle. Cytoplasm|
|Tissue Location||Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes.|
Author : Lan X,Lan X,Chang Y,Zhang X,Liu J,Vikash V,Wang W,Huang M,Wang X,Zhou F,Chen L,Zhao Q.
Cell Physiol Biochem. 2017 Apr 14;41(5):2077-2090. doi: 10.1159/000475439. [Epub ahead of print]
Author : Pinnell N1, Yan R2, Cho HJ3, Keeley T4, Murai MJ3, Liu Y2, Alarcon AS2, Qin J2, Wang Q2, Kuick R5, Elenitoba-Johnson KS6, Maillard I
Author : Rakowski LA1, Garagiola DD, Li CM, Decker M, Caruso S, Jones M, Kuick R, Cierpicki T, Maillard I, Chiang MY.
Cancer Res. 2013 Jan 15;73(2):930-41. doi: 10.1158/0008-5472.CAN-12-1389. Epub 2012 Nov 16.
Provided below are standard protocols that you may find useful for product applications.
Retinoic acid plays a critical role in development, cellular growth, and differentiation and induces the expression of a variety of genes. RAI17 expression is induced by retinoic acid and is predominantly expressed in heart, brain and ovaries. Within brain, highest expression is in amygdala. The deduced 1,067-amino acid protein contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and 2 proline-rich regions. A strong intrinsic transactivation domain is identified in the C-terminal proline-rich region. RAI17 expression is detected in various cancer cell lines. RAI17 colocalizes with endogenous androgen receptor (AR) in the nuclei of prostate epithelial cells from human tissue samples. In human prostate cancer cells, RAI17 increases the transcriptional activity of AR. Studies using sumoylation-deficient AR mutants suggest that the increase of AR activity by RAI17 is dependent upon receptor sumoylation.