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CA IX Monoclonal Antibody(12F10)

     
  • 3 - CA IX Monoclonal Antibody(12F10) AP63330
    Immunofluorescence analysis of human-liver-cancer tissue. 1,CA IX Monoclonal Antibody(12F10)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 0 - CA IX Monoclonal Antibody(12F10) AP63330
    Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CA IX Monoclonal Antibody(12F10) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - CA IX Monoclonal Antibody(12F10) AP63330
    Western blot analysis of 1) Hela, 2) 293T, diluted at 1:5000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
  • 0 - CA IX Monoclonal Antibody(12F10) AP63330
    1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200
  • 0 - CA IX Monoclonal Antibody(12F10) AP63330
    Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 0 - CA IX Monoclonal Antibody(12F10) AP63330
    Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 0 - CA IX Monoclonal Antibody(12F10) AP63330
    Immunohistochemical analysis of paraffin-embedded Human gallbladder. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 0 - CA IX Monoclonal Antibody(12F10) AP63330
    Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 0 - CA IX Monoclonal Antibody(12F10) AP63330
    Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 0 - CA IX Monoclonal Antibody(12F10) AP63330
    Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 0 - CA IX Monoclonal Antibody(12F10) AP63330
    Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:100(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, IP
Primary Accession Q16790
Reactivity Human
Host Mouse
Clonality Monoclonal
Calculated MW 49698 Da
Additional Information
Gene ID 768
Other Names CA9; G250; MN; Carbonic anhydrase 9; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1
Dilution WB~~1:1000
IHC-P~~N/A
IF~~IF: 1:50-200 WB: 1:3000 IP:1:200 IHC 1:50-300
IP~~N/A
Format PBS, pH 7.4, containing 0.09% (W/V) sodium azide as Preservative and 50% Glycerol.
Storage Conditions-20℃
Protein Information
Name CA9
Synonyms G250, MN
Function Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).
Cellular Location Nucleus. Nucleus, nucleolus. Cell membrane; Single-pass type I membrane protein. Cell projection, microvillus membrane; Single-pass type I membrane protein. Note=Found on the surface microvilli and in the nucleus, particularly in nucleolus
Tissue Location Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia.

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