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RPS9 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RPS9 Antibody (C-term) AP6618b
    Western blot analysis of RPS9 antibody (C-term) (Cat. #AP6618b) in Ramos cell line lysates (35ug/lane). RPS9 (arrow) was detected using the purified Pab.
  • 14 - RPS9 Antibody (C-term) AP6618b
    Formalin-fixed and paraffin-embedded human brain tissue reacted with RPS9 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - RPS9 Antibody (C-term) AP6618b
    RPS9 Antibody (C-term) (Cat. #AP6618b) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P46781
Other Accession P29314, Q6ZWN5, A6QLG5
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 22591 Da
Antigen Region 135-164 aa
Additional Information
Gene ID 6203
Other Names 40S ribosomal protein S9, RPS9
Target/Specificity This RPS9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the C-terminal region of human RPS9.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRPS9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name RPS9 (HGNC:10442)
Function Component of the small ribosomal subunit (PubMed:23636399). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (PubMed:23636399). Part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome (PubMed:34516797).
Cellular Location Cytoplasm. Nucleus, nucleolus. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPS9 is a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S4P family of ribosomal proteins. It is located in the cytoplasm.

REFERENCES

Lindstrom,M.S., J. Biol. Chem. 283 (23), 15568-15576 (2008)

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