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VDAC1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - VDAC1 Antibody (N-term) AP6627A
    Anti-VDAC1 Antibody (N-term) at 1:8000 dilution + Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - VDAC1 Antibody (N-term) AP6627A
    Western blot analysis of VDAC1 Antibody (N-term) (Cat. #AP6627a) in A375 cell line and mouse heart tissue lysates (35ug/lane). VDAC1 (arrow) was detected using the purified Pab.
  • 14 - VDAC1 Antibody (N-term) AP6627A
    Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with VDAC1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 1 - VDAC1 Antibody (N-term) AP6627A
    Western blot analysis of VDAC1 (arrow) using rabbit polyclonal VDAC1 Antibody (N-term) (Cat. #AP6627a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the VDAC1 gene.
  • 4 - VDAC1 Antibody (N-term) AP6627A
    VDAC1 Antibody (N-term) (Cat. #AP6627a) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P21796
Other Accession Q9Z2L0, Q9TT15, Q9MZ16, Q60932, P45879
Reactivity Human, Mouse
Predicted Bovine, Pig, Rabbit, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 30773 Da
Additional Information
Gene ID 7416
Other Names Voltage-dependent anion-selective channel protein 1, VDAC-1, hVDAC1, Outer mitochondrial membrane protein porin 1, Plasmalemmal porin, Porin 31HL, Porin 31HM, VDAC1, VDAC
Target/Specificity This VDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human VDAC1.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVDAC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VDAC1
Synonyms VDAC
Function Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterol cholesterol (PubMed:31015432). In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:32047033). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756). May mediate ATP export from cells (PubMed:30061676).
Cellular Location Mitochondrion outer membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Membrane raft; Multi-pass membrane protein
Tissue Location Expressed in erythrocytes (at protein level) (PubMed:27641616). Expressed in heart, liver and skeletal muscle (PubMed:8420959).
Research Areas

BACKGROUND

VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.

REFERENCES

Shoshan-Barmatz,V., Biochim. Biophys. Acta 1787 (5), 421-430 (2009)
Hiller,S., Science 321 (5893), 1206-1210 (2008)

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