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GAS7 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

Anti-GAS7 Antibody (C-term) at 1:1000 dilution + human cerebellum lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of GAS7 antibody (C-term) (Cat. #AP6648b) in A2058 cell line lysates (35ug/lane). GAS7 (arrow) was detected using the purified Pab.
Western blot analysis of GAS7 antibody (C-term) (Cat. #AP6648b) in mouse cerebellum tissue lysates (35ug/lane). GAS7 (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GAS7 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
GAS7 Antibody (C-term) (Cat. #AP6648b) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	O60861
Other Accession	O55148, Q60780
Reactivity	Human, Mouse
Predicted	Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	54371 Da
Antigen Region	438-465 aa

Additional Information
Gene ID	8522
Other Names	Growth arrest-specific protein 7, GAS-7, GAS7, KIAA0394
Target/Specificity	This GAS7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-465 amino acids from the C-terminal region of human GAS7.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GAS7 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GAS7
Synonyms	KIAA0394
Function	May play a role in promoting maturation and morphological differentiation of cerebellar neurons.
Cellular Location	Cytoplasm.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development.

REFERENCES

Chao,C.C., J. Neurosci. Res. 81 (2), 153-162 (2005)
She,B.R., Exp. Cell Res. 273 (1), 34-44 (2002)

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¥ 1,250.00

Cat# AP6648B

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¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

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询价

GAS7 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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