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ZWINT Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ZWINT Antibody (Center) AP6686c
    Anti-ZWINT Antibody (Center) at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ZWINT Antibody (Center) AP6686c
    Western blot analysis of ZWINT antibody (Center) (Cat. #AP6686c) in mouse cerebellum tissue lysates (35ug/lane). ZWINT (arrow) was detected using the purified Pab.
  • 14 - ZWINT Antibody (Center) AP6686c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with ZWINT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - ZWINT Antibody (Center) AP6686c
    Flow cytometric analysis of widr cells using ZWINT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • 1 - ZWINT Antibody (Center) AP6686c
    Western blot analysis of ZWINT (arrow) using rabbit polyclonal ZWINT Antibody (Center) (Cat. #AP6686c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ZWINT gene.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession O95229
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 31293 Da
Additional Information
Gene ID 11130
Other Names ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT
Target/Specificity This ZWINT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the Central region of human ZWINT.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsZWINT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZWINT
Function Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
Cellular Location Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase
Research Areas

BACKGROUND

ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.

REFERENCES

Famulski,J.K., J. Cell Biol. 180 (3), 507-520 (2008) Kops,G.J., J. Cell Biol. 169 (1), 49-60 (2005) Wang,H., J. Biol. Chem. 279 (52), 54590-54598 (2004)

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