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|Application ||WB, IHC-P, FC, E|
|Calculated MW||31293 Da|
|Other Names||ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT|
|Target/Specificity||This ZWINT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the Central region of human ZWINT.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ZWINT Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.|
|Cellular Location||Nucleus. Chromosome, centromere, kinetochore. Note=Localizes to kinetochores from late prophase to anaphase|
Provided below are standard protocols that you may find useful for product applications.
ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells.
Famulski,J.K., J. Cell Biol. 180 (3), 507-520 (2008) Kops,G.J., J. Cell Biol. 169 (1), 49-60 (2005) Wang,H., J. Biol. Chem. 279 (52), 54590-54598 (2004)