Fascin-3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

Anti-Fascin-3 Antibody (N-term) at 1:8000 dilution + human testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 57 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of Fascin-3 Antibody (N-term) (Cat. #AP6757c) in MDA-MB231, NCI-H460 cell line and mouse testis tissue lysates (35ug/lane). Fascin-3 (arrow) was detected using the purified Pab.
Fascin-3 Antibody (N-term) (Cat. #AP6757c) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Fascin-3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Fascin-3 Antibody (N-term) (Cat. #AP6757c) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q9NQT6
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	56624 Da
Antigen Region	1-30 aa

Additional Information
Gene ID	29999
Other Names	Fascin-3, Testis fascin, FSCN3
Target/Specificity	This Fascin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Fascin-3.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Fascin-3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.