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>   首页   >   产品   >   一抗   >   心血管   >   Calponin-1 Antibody (N-term)   

Calponin-1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Calponin-1 Antibody (N-term) AP6795A
    Western blot analysis of Calponin-1 Antibody (N-term) (Cat. #AP6795a) in NCI-H460 cell line lysates (35ug/lane). CNN1 (arrow) was detected using the purified Pab.
  • 14 - Calponin-1 Antibody (N-term) AP6795A
    Formalin-fixed and paraffin-embedded human lung carcinoma with Calponin-1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - Calponin-1 Antibody (N-term) AP6795A
    Flow cytometric analysis of NCI-H292 cells using Calponin-1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P51911
Other Accession Q08290, Q08091, Q2HJ38
Reactivity Human
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 33170 Da
Antigen Region 10-39 aa
Additional Information
Gene ID 1264
Other Names Calponin-1, Basic calponin, Calponin H1, smooth muscle, CNN1
Target/Specificity This Calponin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-39 amino acids from the N-terminal region of human Calponin-1.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCalponin-1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CNN1
Function Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).
Tissue Location Smooth muscle, and tissues containing significant amounts of smooth muscle
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

REFERENCES

Marsh,W.L., et.al., Appl. Immunohistochem. Mol. Morphol. 17 (3), 216-219 (2009)

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