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ATF-1 Polyclonal Antibody

     
  • 1 - ATF-1 Polyclonal Antibody AP68562
    Western Blot analysis of various cells using ATF-1 Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
  • 0 - ATF-1 Polyclonal Antibody AP68562
    Western blot analysis of 293T 3T3 HELA KB SH-SY5Y lysis using ATF-1 antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF
Primary Accession P18846
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 29233 Da
Additional Information
Gene ID 466
Other Names ATF1; Cyclic AMP-dependent transcription factor ATF-1; cAMP-dependent transcription factor ATF-1; Activating transcription factor 1; Protein TREB36
Dilution WB~~Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
IHC-P~~N/A
IF~~1:50~200
Format Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions-20℃
Protein Information
Name ATF1
Function This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Cellular Location Nucleus.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.

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