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ETFDH Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ETFDH Antibody (N-term) AP6877a
    Anti-ETFDH Antibody (N-term) at 1:1000 dilution + H. kidney whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - ETFDH Antibody (N-term) AP6877a
    Formalin-fixed and paraffin-embedded human lung carcinoma reacted with ETFDH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - ETFDH Antibody (N-term) AP6877a
    ETFDH Antibody (N-term) (Cat. #AP6877a) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, FC, WB, E
Primary Accession Q16134
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 68495 Da
Antigen Region 32-61 aa
Additional Information
Gene ID 2110
Other Names Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial, ETF-QO, ETF-ubiquinone oxidoreductase, Electron-transferring-flavoprotein dehydrogenase, ETF dehydrogenase, ETFDH
Target/Specificity This ETFDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human ETFDH.
Dilution IHC-P~~1:100~500
FC~~1:10~50
WB~~1:1000
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsETFDH Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ETFDH (HGNC:3483)
Function Accepts electrons from ETF and reduces ubiquinone.
Cellular Location Mitochondrion inner membrane.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Electron-transferring-flavoprotein dehydrogenase in the inner mitochondrial membrane accepts electrons from electron-transfer flavoprotein which is located in the mitochondrial matrix and reduces ubiquinone in the mitochondrial membrane. The protein is synthesized as a 67-kDa precursor which is targeted to mitochondria and processed in a single step to a 64-kDa mature form located in the mitochondrial membrane. Deficiency in electron-transferring-flavoprotein dehydrogenase have been demonstrated in some patients with type II glutaricacidemia.

REFERENCES

Olsen,R.K., et.al., Hum. Mutat. 22 (1), 12-23 (2003)

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