CDK5 Activator-binding C42 Polyclonal Antibody

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P
Primary Accession	Q96SZ6
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	67689 Da

Additional Information
Gene ID	51654
Other Names	CDK5RAP1; C20orf34; CGI-05; HSPC167; CDK5 regulatory subunit-associated protein 1; CDK5 activator-binding protein C42
Dilution	IHC-P~~N/A
Format	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions	-20℃

Protein Information
Name	CDK5RAP1 (HGNC:15880)
Synonyms	C20orf34
Function	Methylthiotransferase that catalyzes the conversion of N6- (dimethylallyl)adenosine (i(6)A) to 2-methylthio-N6- (dimethylallyl)adenosine (ms(2)i(6)A) at position 37 (adjacent to the 3'-end of the anticodon) of four mitochondrial DNA-encoded tRNAs (Ser(UCN), Phe, Tyr and Trp) (PubMed:22422838, PubMed:25738458, PubMed:28981754). Essential for efficient and highly accurate protein translation by the ribosome (PubMed:22422838, PubMed:25738458, PubMed:28981754). Specifically inhibits CDK5 activation by CDK5R1 (PubMed:11882646). Essential for efficient mitochondrial protein synthesis and respiratory chain; shows pathological consequences in mitochondrial disease (PubMed:25738458).
Cellular Location	Mitochondrion.
Tissue Location	Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas (PubMed:10721722). Expressed in neurons of central nervous tissue (PubMed:10721722, PubMed:11882646) [Isoform 6]: High expression in placenta and lung.