CLC-4 Polyclonal Antibody

Western Blot analysis of various cells using CLC-4 Polyclonal Antibody diluted at 1：500
Western Blot analysis of RAT-MUSLE cells using CLC-4 Polyclonal Antibody diluted at 1：500

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF
Primary Accession	P51793
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	84917 Da

Additional Information
Gene ID	1183
Other Names	CLCN4; H(+)/Cl(-) exchange transporter 4; Chloride channel protein 4; ClC-4; Chloride transporter ClC-4
Dilution	WB~~Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications. IF~~1:50~200
Format	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions	-20℃

Protein Information
Name	CLCN4
Function	Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:18063579, PubMed:23647072, PubMed:25644381, PubMed:27550844, PubMed:28972156). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (PubMed:29845874). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (PubMed:29845874).
Cellular Location	Early endosome membrane {ECO:0000250\|UniProtKB:P51794}; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Recycling endosome membrane; Multi-pass membrane protein. Note=Localizes to late endosome membrane, lysosome membrane and recycling endosome membrane in the presence of CLCN3
Tissue Location	Abundant in skeletal muscle and also detectable in brain and heart