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NDRG1 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - NDRG1 Antibody (N-term) AP6935a
    Western blot analysis of NDRG1 Antibody (N-term) (Cat. #AP6935a) in MDA-MB231 cell line lysates (35ug/lane). NDRG1 (arrow) was detected using the purified Pab.
  • 14 - NDRG1 Antibody (N-term) AP6935a
    Formalin-fixed and paraffin-embedded human prostate carcinoma with NDRG1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 4 - NDRG1 Antibody (N-term) AP6935a
    Flow cytometric analysis of MDA-231 cells using NDRG1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q92597
Other Accession Q6JE36, Q62433, Q4R4Q3, Q3SYX0
Reactivity Human
Predicted Bovine, Monkey, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Antigen Region 12-40 aa
Additional Information
Other Names Protein NDRG1, Differentiation-related gene 1 protein, DRG-1, N-myc downstream-regulated gene 1 protein, Nickel-specific induction protein Cap43, Reducing agents and tunicamycin-responsive protein, RTP, Rit42, NDRG1, CAP43, DRG1, RTP
Target/Specificity This NDRG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human NDRG1.
Dilution WB~~1:1000
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNDRG1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

NDRG1 is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. It is necessary for p53-mediated caspase activation and apoptosis.

REFERENCES

Sugiyama,N., et.al., Mol. Cell Proteomics 6 (6), 1103-1109 (2007)

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