GCP5 Polyclonal Antibody

Western Blot analysis of various cells using GCP5 Polyclonal Antibody diluted at 1：2000
Western Blot analysis of 293 cells using GCP5 Polyclonal Antibody diluted at 1：2000
Western blot analysis of Mouse-kidney mouse-brain HELA KB SH-SY5Y 293T lysis using GCP5 antibody. Antibody was diluted at 1:2000
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF, ICC, E
Primary Accession	Q96RT8
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	118321 Da

Additional Information
Gene ID	114791
Other Names	TUBGCP5; GCP5; KIAA1899; Gamma-tubulin complex component 5; GCP-5
Dilution	WB~~Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. IHC-P~~Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. IF~~1:50~200 ICC~~N/A E~~N/A
Format	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions	-20℃

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	TUBGCP5
Synonyms	GCP5, KIAA1899
Function	Component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation (PubMed:38305685, PubMed:38609661, PubMed:39321809). The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation (PubMed:38305685, PubMed:38609661, PubMed:39321809).
Cellular Location	Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Tissue Location	Widely expressed, with highest levels in heart and skeletal muscle and moderate levels in brain