癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GRK4 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
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- 文献引用 : 1
- 实验流程
- 背景知识
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | P32298 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 66583 Da |
| Antigen Region | 549-577 aa |
| Gene ID | 2868 |
|---|---|
| Other Names | G protein-coupled receptor kinase 4, G protein-coupled receptor kinase GRK4, ITI1, GRK4, GPRK2L, GPRK4 |
| Target/Specificity | This GRK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-577 amino acids from the C-terminal region of human GRK4. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | GRK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | GRK4 |
|---|---|
| Synonyms | GPRK2L, GPRK4 |
| Function | Specifically phosphorylates the activated forms of G protein- coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2. |
| Cellular Location | Cytoplasm. Cytoplasm, cell cortex. Note=Both at the cell surface and dispersed in the cytoplasm under basal conditions Receptor stimulation results in the internalization of GRK4 to the perinuclear area, where colocalization with DRD3 is observed strongly at 5 and 15 minutes. DRD3 and GRK4 colocalize in lipid rafts of renal proximal tubule cells |
| Tissue Location | Isoform 1, isoform 2, isoform 3, and isoform 4 are expressed in testis. Isoform 4 is expressed in myometrium |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
REFERENCES
Perroy, J., et al., EMBO J. 22(15):3816-3824 (2003).
Watanabe, H., et al., Kidney Int. 62(3):790-798 (2002).
Sallese, M., et al., J. Biol. Chem. 272(15):10188-10195 (1997).
Premont, R.T., et al., J. Biol. Chem. 271(11):6403-6410 (1996).
Sallese, M., et al., Biochem. Biophys. Res. Commun. 199(2):848-854 (1994).
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