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IκB-α Polyclonal Antibody

     
  • 3 - IκB-α Polyclonal Antibody AP70600
    Immunofluorescence analysis of rat-lung tissue. 1,IκB-α Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunofluorescence analysis of rat-lung tissue. 1,IκB-α Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunofluorescence analysis of rat-spleen tissue. 1,IκB-α Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunofluorescence analysis of rat-spleen tissue. 1,IκB-α Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunofluorescence analysis of mouse-kidney tissue. 1,IκB-α Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunofluorescence analysis of mouse-kidney tissue. 1,IκB-α Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 0 - IκB-α Polyclonal Antibody AP70600
    Western Blot analysis of various cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Mouse-colon tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Immunohistochemical analysis of paraffin-embedded Mouse-spleen tissue. 1,IκB-α Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 0 - IκB-α Polyclonal Antibody AP70600
    Western Blot analysis of various cells using IκB-α Polyclonal Antibody diluted at 1:2000
  • 0 - IκB-α Polyclonal Antibody AP70600
    Western Blot analysis of 293 cells using IκB-α Polyclonal Antibody diluted at 1:2000
  • 0 - IκB-α Polyclonal Antibody AP70600
    Western Blot analysis of 293 K562 HELA cells using IκB-α Polyclonal Antibody diluted at 1:2000
  • 0 - IκB-α Polyclonal Antibody AP70600
    Western blot analysis of customer's sample lysis using IκB-α antibody. Antibody was diluted at 1:2000
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF
Primary Accession P25963
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 35609 Da
Additional Information
Gene ID 4792
Other Names NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3
Dilution WB~~1:1000
IHC-P~~N/A
IF~~IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
Format Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions-20℃
Protein Information
Name NFKBIA
Synonyms IKBA, MAD3, NFKBI
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:36651806, PubMed:7479976). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7479976, PubMed:7628694, PubMed:7796813, PubMed:7878466).
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

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