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PGK1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 14 - PGK1 Antibody (Center) AP7094b
    Immunohistochemical analysis of paraffin-embedded H. kidney section using PGK1 Antibody (Center)(Cat#AP7094b). AP7094b was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
  • 1 - PGK1 Antibody (Center) AP7094b
    Western blot analysis of lysates from Hela, U-937 cell line (from left to right), using PGK1 Antibody (G132)(Cat. #AP7094b). AP7094b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
  • 14 - PGK1 Antibody (Center) AP7094b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 3 - PGK1 Antibody (Center) AP7094b
    Confocal immunofluorescent analysis of PGK1 Antibody (Center)(Cat#AP7094b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
  • 4 - PGK1 Antibody (Center) AP7094b
    PGK1 Antibody (Center) (Cat. #AP7094b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, FC, WB, E
Primary Accession P00558
Other Accession P16617, P09411, Q60HD8
Reactivity Human, Mouse
Predicted Monkey, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44615 Da
Additional info
Gene ID 5230
Other Names Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA
Target/Specificity This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the Central region of human PGK1.
Dilution IHC-P~~1:10~50
WB~~1:1000
IF~~1:10~50
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPGK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PGK1
Synonyms PGKA
Function In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
Cellular Location Cytoplasm.
Tissue Location Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men
Research Areas
The effect of 3-bromopyruvate on human colorectal cancer cells is dependent on glucose concentration but not hexokinase II expression.
Author : Ho N1, Morrison J1, Silva A1, Coomber BL2.
Biosci Rep. 2016 Jan 6. pii: BSR20150267. [Epub ahead of print]
26740252
Antrodia cinnamomea Inhibits Migration in Human Hepatocellular Carcinoma Cells.
Author : Chen YY1, Liu FC1, Wu TS2, Sheu MJ1.
Am J Chin Med. 2015 Nov 30:1550095. [Epub ahead of print]
26621448
Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis.
Author : Xu W, Fang P, Zhu Z, Dai J, Nie D, Chen Z, Qin Q, Wang L, Wang Z, Qiao Z.
Biol Reprod. 2013 Dec 19;89(6):142. doi: 10.1095/biolreprod.113.111245. Print 2013.
24198121
Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice.
Author : Danshina PV, Geyer CB, Dai Q, Goulding EH, Willis WD, Kitto GB, McCarrey JR, Eddy EM, O'Brien DA.
Biol Reprod. 2010 Jan;82(1):136-45. doi: 10.1095/biolreprod.109.079699. Epub 2009 Sep 16.
19759366
Opposite pathobiochemical fate of pyruvate kinase and adenylate kinase in aged rat skeletal muscle as revealed by proteomic DIGE analysis.
Author : Doran P, O'Connell K, Gannon J, Kavanagh M, Ohlendieck K.
Proteomics. 2008 Jan;8(2):364-77.
18050275
Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle.
Author : O'Connell K, Gannon J, Doran P, Ohlendieck K.
Int J Mol Med. 2007 Aug;20(2):145-53.
17611631
Role of hypoxia inducible factor-1 alpha in modulation of apoptosis resistance.
Author : Kilic M, Kasperczyk H, Fulda S, Debatin KM.
Oncogene. 2007 Mar 29;26(14):2027-38. Epub 2006 Oct 16.
17043658
Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity.
Author : West AB, Moore DJ, Biskup S, Bugayenko A, Smith WW, Ross CA, Dawson VL, Dawson TM.
Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16842-7. Epub 2005 Nov 3.
16269541

BACKGROUND

Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

REFERENCES

Lay, A. J., et al. Nature 408: 869-873 (2000).

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