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PGK1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

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Immunohistochemical analysis of paraffin-embedded H. kidney section using PGK1 Antibody (Center)(Cat#AP7094b). AP7094b was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysates from Hela, U-937 cell line (from left to right), using PGK1 Antibody (G132)(Cat. #AP7094b). AP7094b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PGK1 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of PGK1 Antibody (Center)(Cat#AP7094b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
PGK1 Antibody (Center) (Cat. #AP7094b) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

产品详情
文献引用 : 8
实验流程
背景知识

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IF, FC, WB, E
Primary Accession	P00558
Other Accession	P16617, P09411, Q60HD8
Reactivity	Human, Mouse
Predicted	Monkey, Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig
Calculated MW	44615 Da

Additional info
Gene ID	5230
Other Names	Phosphoglycerate kinase 1, Cell migration-inducing gene 10 protein, Primer recognition protein 2, PRP 2, PGK1, PGKA
Target/Specificity	This PGK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 117-145 amino acids from the Central region of human PGK1.
Dilution	IHC-P~~1:10~50 WB~~1:1000 IF~~1:10~50 FC~~1:10~50
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	PGK1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	PGK1
Synonyms	PGKA
Function	In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) (PubMed:2324090). May play a role in sperm motility (PubMed:26677959).
Cellular Location	Cytoplasm.
Tissue Location	Mainly expressed in spermatogonia. Localized on the principle piece in the sperm (at protein level). Expression significantly decreased in the testis of elderly men

Research Areas

The effect of 3-bromopyruvate on human colorectal cancer cells is dependent on glucose concentration but not hexokinase II expression.
Author : Ho N¹, Morrison J¹, Silva A¹, Coomber BL². Biosci Rep. 2016 Jan 6. pii: BSR20150267. [Epub ahead of print]	26740252

Antrodia cinnamomea Inhibits Migration in Human Hepatocellular Carcinoma Cells.
Author : Chen YY¹, Liu FC¹, Wu TS², Sheu MJ¹. Am J Chin Med. 2015 Nov 30:1550095. [Epub ahead of print]	26621448

Cigarette smoking exposure alters pebp1 DNA methylation and protein profile involved in MAPK signaling pathway in mice testis.
Author : Xu W, Fang P, Zhu Z, Dai J, Nie D, Chen Z, Qin Q, Wang L, Wang Z, Qiao Z. Biol Reprod. 2013 Dec 19;89(6):142. doi: 10.1095/biolreprod.113.111245. Print 2013.	24198121

Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice.
Author : Danshina PV, Geyer CB, Dai Q, Goulding EH, Willis WD, Kitto GB, McCarrey JR, Eddy EM, O'Brien DA. Biol Reprod. 2010 Jan;82(1):136-45. doi: 10.1095/biolreprod.109.079699. Epub 2009 Sep 16.	19759366

Opposite pathobiochemical fate of pyruvate kinase and adenylate kinase in aged rat skeletal muscle as revealed by proteomic DIGE analysis.
Author : Doran P, O'Connell K, Gannon J, Kavanagh M, Ohlendieck K. Proteomics. 2008 Jan;8(2):364-77.	18050275

Proteomic profiling reveals a severely perturbed protein expression pattern in aged skeletal muscle.
Author : O'Connell K, Gannon J, Doran P, Ohlendieck K. Int J Mol Med. 2007 Aug;20(2):145-53.	17611631

Role of hypoxia inducible factor-1 alpha in modulation of apoptosis resistance.
Author : Kilic M, Kasperczyk H, Fulda S, Debatin KM. Oncogene. 2007 Mar 29;26(14):2027-38. Epub 2006 Oct 16.	17043658

Parkinson's disease-associated mutations in leucine-rich repeat kinase 2 augment kinase activity.
Author : West AB, Moore DJ, Biskup S, Bugayenko A, Smith WW, Ross CA, Dawson VL, Dawson TM. Proc Natl Acad Sci U S A. 2005 Nov 15;102(46):16842-7. Epub 2005 Nov 3.	16269541

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), this major enzyme in glycolysis catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, generating one molecule of ATP. New blood vessel formation or angiogenesis is critical for tumor expansion and metastasis. Lay et al. (2000) showed that the plasmin reductase isolated from conditioned medium of fibrosarcoma cells is the glycolytic enzyme phosphoglycerate kinase. They concluded that phosphoglycerate kinase not only functions in glycolysis but is secreted by tumor cells and participates in the angiogenic process as a disulfide reductase.

REFERENCES

Lay, A. J., et al. Nature 408: 869-873 (2000).

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Cat# AP7094b

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¥ 1,100.00

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PGK1 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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