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Mlx Polyclonal Antibody

     
  • 1 - Mlx Polyclonal Antibody AP70974
    Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°,overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P
Primary Accession Q9UH92
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 33300 Da
Additional Information
Gene ID 6945
Other Names MLX; BHLHD13; TCFL4; Max-like protein X; Class D basic helix-loop-helix protein 13; bHLHd13; Max-like bHLHZip protein; Protein BigMax; Transcription factor-like protein 4
Dilution WB~~Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
IHC-P~~N/A
Format Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions-20℃
Protein Information
Name MLX
Synonyms BHLHD13, TCFL4
Function Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
Cellular Location [Isoform Alpha]: Cytoplasm. Note=Found predominantly in the cytoplasm (PubMed:10918583). [Isoform Gamma]: Nucleus. Note=Found predominantly in the nucleus (PubMed:10918583).
Tissue Location Expressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.

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