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>   首页   >   产品   >   一抗   >   其他   >   NOS3 Polyclonal Antibody   

NOS3 Polyclonal Antibody

     
  • 1 - NOS3 Polyclonal Antibody AP71357
    Immunofluorescence analysis of rat-lung tissue. 1,NOS3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunofluorescence analysis of rat-lung tissue. 1,NOS3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunofluorescence analysis of rat-kidney tissue. 1,NOS3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunofluorescence analysis of rat-kidney tissue. 1,NOS3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunofluorescence analysis of rat-spleen tissue. 1,NOS3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunofluorescence analysis of rat-spleen tissue. 1,NOS3 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 14 - NOS3 Polyclonal Antibody AP71357
    Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,NOS3 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 1 - NOS3 Polyclonal Antibody AP71357
    Western Blot analysis of A549 cells using NOS3 Polyclonal Antibody diluted at 1:1000
  • 1 - NOS3 Polyclonal Antibody AP71357
    Western Blot analysis of A549 cells using NOS3 Polyclonal Antibody diluted at 1:1000
  • 1 - NOS3 Polyclonal Antibody AP71357
    Western blot analysis of mouse-brain lysis using NOS3 antibody. Antibody was diluted at 1:1000
  • 1 - NOS3 Polyclonal Antibody AP71357
    Western blot analysis of mouse-brain lysis using NOS3 antibody. Antibody was diluted at 1:1000
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF
Primary Accession P29474
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 133275 Da
Additional Information
Gene ID 4846
Other Names NOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII
Dilution WB~~WB 1:500-2000, IF 1:50-300, IHC 1:50-300
IHC-P~~WB 1:500-2000, IF 1:50-300, IHC 1:50-300
IF~~1:50~200
Format Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions-20℃
Protein Information
Name NOS3 (HGNC:7876)
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway (PubMed:1378832). NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Cellular Location Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity
Tissue Location Platelets, placenta, liver and kidney.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

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