HIPK4 Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

Anti-HIPK4 C-term at 1:1000 dilution + H. testis whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of HIPK4 C-term (Cat. #AP7138b) in HL-60 cell line lysates (35ug/lane). HIPK4 (arrow) was detected using the purified Pab.
Western blot analysis of HIPK4 (arrow) using rabbit polyclonal HIPK4 Antibody (C-term) (Cat. #AP7138b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HIPK4 gene.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	Q8NE63
Other Accession	Q8WP28
Reactivity	Human
Predicted	Monkey, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	69425 Da
Antigen Region	537-566 aa

Additional Information
Gene ID	147746
Other Names	Homeodomain-interacting protein kinase 4, HIPK4
Target/Specificity	This HIPK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-566 amino acids from the C-terminal region of human HIPK4.
Dilution	WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	HIPK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	HIPK4
Function	Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors (Potential).
Cellular Location	Cytoplasm.

Research Areas