HIPK4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

Anti-HIPK4 C-term at 1:1000 dilution + H. testis whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot analysis of HIPK4 C-term (Cat. #AP7138b) in HL-60 cell line lysates (35ug/lane). HIPK4 (arrow) was detected using the purified Pab.
Western blot analysis of HIPK4 (arrow) using rabbit polyclonal HIPK4 Antibody (C-term) (Cat. #AP7138b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HIPK4 gene.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	Q8NE63
Other Accession	Q8WP28
Reactivity	Human
Predicted	Monkey
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	69425 Da
Antigen Region	537-566 aa

Additional Information
Gene ID	147746
Other Names	Homeodomain-interacting protein kinase 4, HIPK4
Target/Specificity	This HIPK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-566 amino acids from the C-terminal region of human HIPK4.
Dilution	WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	HIPK4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	HIPK4
Function	Protein kinase that phosphorylates human TP53 at Ser-9, and thus induces TP53 repression of BIRC5 promoter (By similarity). May act as a corepressor of transcription factors (Potential).
Cellular Location	Cytoplasm.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.