- 文献引用 : 5
|Application ||WB, IHC-P, E|
|Other Accession||O70444, P58750|
|Calculated MW||35891 Da|
|Other Names||Serine/threonine-protein kinase pim-3, PIM3|
|Target/Specificity||This PIM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-288 amino acids from the C-terminal region of human PIM3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PIM3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis, promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti- apoptotic protein Bcl-X(L), the regulation of cell cycle progression, protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.|
|Tissue Location||Detected in various tissues, including the heart, brain, lung, kidney, spleen, placenta, skeletal muscle, and peripheral blood leukocytes. Not found or barely expressed in the normal adult endoderm-derived organs such as colon, thymus, liver, or small intestine. However, expression is augmented in premalignant and malignant lesions of these organs.|
Provided below are standard protocols that you may find useful for product applications.
PIM3 is a memberof the Ser/Thr protein kinase family that may be involved in cell cycle progression and anti-apoptosis process. This protein has been implicated in proliferation of human hepatoma cell lines. PIM3 is widely expressed, with the exception of no expression observed in colon, thymus, and small intestine. PIM3 is expressed in human hepatoma cell lines but not in normal liver tissues.
Mikkers, H., et al., Mol. Cell. Biol. 24(13):6104-6115 (2004). Eichmann, A., et al., Oncogene 19(9):1215-1224 (2000).