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>   首页   >   产品   >   一抗   >   神经科学   >   CAMK1G Antibody (Center K226)   

CAMK1G Antibody (Center K226)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - CAMK1G Antibody (Center K226) AP7253c
    CAMK1G Antibody (Center K226) (Cat.#AP7253c) western blot analysis in HL-60,K562 cell line and mouse cerebellum tissue lysates (35ug/lane).This demonstrates the CAMK1G antibody detected the CAMK1G protein (arrow).
  • 1 - CAMK1G Antibody (Center K226) AP7253c
    Western blot analysis of CAMK1G (arrow) using rabbit polyclonal CAMK1G (Center K226) Antibody (Cat.#AP7253c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with CAMK1G gene (Lane 2) (Origene Technologies).
  • 14 - CAMK1G Antibody (Center K226) AP7253c
    Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CAMK1G (Center K226) (Cat.#AP7253c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q96NX5
Other Accession Q7TNJ7, Q91VB2, NP_065172
Reactivity Human
Predicted Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 53087 Da
Antigen Region 211-241 aa
Additional Information
Gene ID 57172
Other Names Calcium/calmodulin-dependent protein kinase type 1G, CaM kinase I gamma, CaM kinase IG, CaM-KI gamma, CaMKI gamma, CaMKIG, CaMK-like CREB kinase III, CLICK III, CAMK1G, CLICK3, VWS1
Target/Specificity This CAMK1G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-241 amino acids from the Central region of human CAMK1G.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCAMK1G Antibody (Center K226) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CAMK1G
Synonyms CLICK3, VWS1
Function Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates transcription factor CREB1 (By similarity).
Cellular Location Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Cell membrane; Peripheral membrane protein
Tissue Location Mainly expressed in brain with small amounts in skeletal muscles, kidney, spleen and liver. Strongly expressed in forebrain neocortex, striatum and limbic system
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Ca2+/calmodulin-dependent protein kinase I (CaMKI) constitutes a family of closely related isoforms (alpha, beta and gamma). CLICK-III/CaMKIgamma is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase. AMKIgamma is abundant in neurons, particularly in the amygdala and ventromedial hypothalamus. Like the other CaMKI isoforms, full activation of CLICK-III/CaMKIgamma requires both Ca(2+)/CaM and phosphorylation by CaMKK.

REFERENCES

Takemoto-Kimura, S., et al., J. Biol. Chem. 278(20):18597-18605 (2003).
Schutte, B.C., et al., Genome Res. 10(1):81-94 (2000).

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