CD73 Polyclonal Antibody

Western Blot analysis of MCF7, L929, K562 cells using CD73 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000
Immunohistochemical analysis of paraffin-embedded human-skin, antibody was diluted at 1:100

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P
Primary Accession	P21589
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	63368 Da

Additional Information
Gene ID	4907
Other Names	NT5E; NT5; NTE; 5'-nucleotidase; 5'-NT; Ecto-5'-nucleotidase; CD73
Dilution	WB~~Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications. IHC-P~~N/A
Format	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions	-20℃

Protein Information
Name	NT5E
Synonyms	NT5, NTE
Function	Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:22997138, PubMed:23142347, PubMed:24887587, PubMed:34403084).
Cellular Location	Cell membrane; Lipid-anchor, GPI-anchor