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EPAS-1 Polyclonal Antibody

     
  • 3 - EPAS-1 Polyclonal Antibody AP73508
    Immunofluorescence analysis of human-stomach tissue. 1,EPAS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunofluorescence analysis of human-stomach tissue. 1,EPAS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunofluorescence analysis of mouse-spleen tissue. 1,EPAS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunofluorescence analysis of mouse-spleen tissue. 1,EPAS-1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,EPAS-1 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
  • 1 - EPAS-1 Polyclonal Antibody AP73508
    Western Blot analysis of Jurkat, L929 cells using EPAS-1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded human-mammary-cancer, antibody was diluted at 1:100
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
  • 14 - EPAS-1 Polyclonal Antibody AP73508
    Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:100
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF
Primary Accession Q99814
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 96459 Da
Additional Information
Gene ID 2034
Other Names EPAS1; BHLHE73; HIF2A; MOP2; PASD2; Endothelial PAS domain-containing protein 1; EPAS-1; Basic-helix-loop-helix-PAS protein MOP2; Class E basic helix-loop-helix protein 73; bHLHe73;HIF-1-alpha-like factor; HLF; Hypoxia-inducible factor 2-alpha; HIF-2-alpha; HIF2-alpha; Member of PAS protein 2; PAS domain-containing protein 2
Dilution WB~~IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
IHC-P~~IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
IF~~IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.
Format Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions-20℃
Protein Information
Name EPAS1
Synonyms BHLHE73, HIF2A, MOP2, PASD2
Function Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD (By similarity).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P97481, ECO:0000255|PROSITE-ProRule:PRU00981}. Nucleus speckle {ECO:0000250|UniProtKB:P97481}. Note=Colocalizes with HIF3A in the nucleus and speckles. {ECO:0000250|UniProtKB:P97481}
Tissue Location Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (By similarity). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity).

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