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Bob 1 Polyclonal Antibody

     
  • 14 - Bob 1 Polyclonal Antibody AP74165
    Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200
  • 14 - Bob 1 Polyclonal Antibody AP74165
    Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 14 - Bob 1 Polyclonal Antibody AP74165
    Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 14 - Bob 1 Polyclonal Antibody AP74165
    Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
  • 14 - Bob 1 Polyclonal Antibody AP74165
    Immunohistochemical analysis of paraffin-embedded Human spleen. 1, Antibody was diluted at 1:200(4°,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IF, ICC, E
Primary Accession Q16633
Reactivity Human, Rat, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 27436 Da
Additional Information
Gene ID 5450
Other Names POU domain class 2-associating factor 1 (B-cell-specific coactivator OBF-1) (BOB-1) (OCA-B) (OCT-binding factor 1)
Dilution IHC-P~~IHC-p 1:50-200, ELISA 1:10000-20000
IF~~1:50~200
ICC~~N/A
E~~N/A
Format Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.09% (W/V) sodium azide.
Storage Conditions-20℃

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name POU2AF1 (HGNC:9211)
Function Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2 (PubMed:7859290). It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2 (PubMed:7779176). It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2 (PubMed:7779176). It is essential for the response of B-cells to antigens and required for the formation of germinal centers (PubMed:7623806, PubMed:7859290). Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator (By similarity).
Cellular Location Nucleus.
Tissue Location B-cell specific (PubMed:7779176, PubMed:7859290). Detected in mainly in spleen, but also in thymus, periphral blood leukocyte and small intestine (PubMed:7779176, PubMed:7859290)
Research Areas

BACKGROUND

Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

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