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ANXA2 Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of lysates from A431, SK-BR-3 cell line (from left to right), using ANXA2 Antibody (C-term)(Cat. #AP7420b). AP7420b was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody (C-term) (Cat.#AP7420b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of ANXA2 Antibody (C-term)(Cat#AP7420b) with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IF, WB, E
Primary Accession	P07355
Other Accession	A6NMY6, Q07936, P19620, P07356
Reactivity	Human
Predicted	Mouse, Pig, Rat, Bovine, Canine, Rabbit, Chicken
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Antigen Region	287-313 aa

Additional Information
Other Names	Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, PAP-IV, Protein I, p36, ANXA2, ANX2, ANX2L4, CAL1H, LPC2D
Target/Specificity	This ANXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-313 amino acids from the C-terminal region of human ANXA2.
Dilution	IHC-P~~1:100~500 IF~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ANXA2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

ANXA2 is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.

REFERENCES

He,K.L., J. Biol. Chem. 283 (28), 19192-19200 (2008)
Tamma,G., Pflugers Arch. 456 (4), 729-736 (2008)
Gou,D., J. Biol. Chem. 283 (19), 13156-13164 (2008)

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¥ 1,250.00

Cat# AP7420b

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¥ 1,250.00

¥ 2,050.00

¥ 2,960.00

¥ 4,550.00

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ANXA2 Antibody (C-term) 精选

Purified Rabbit Polyclonal Antibody (Pab)

BACKGROUND

REFERENCES

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