注册 | 登录
点击这里给我发消息
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   心血管   >   VINC Antibody (N-term)   

VINC Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - VINC Antibody (N-term) AP7426a
    All lanes : Anti-VINC Antibody (N-term) at 1:2000 dilution Lane 1: RD whole cell lysates Lane 2: Hela whole cell lysates Lane 3: rat liver lysates Lane 4: human testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 124 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - VINC Antibody (N-term) AP7426a
    Western blot analysis of anti-VINC Antibody (N-term) (Cat.#AP7426a) in K562 cell line lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.
  • 1 - VINC Antibody (N-term) AP7426a
    Western blot analysis of anti-VINC Antibody (N-term) (Cat.#AP7426a) in mouse lung tissue lysates (35ug/lane). VINC (arrow) was detected using the purified Pab.
  • 14 - VINC Antibody (N-term) AP7426a
    Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with VINC antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 产品详情
  • 实验流程
  • 背景知识
Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P18206
Other Accession P85972, P26234, Q64727, P12003
Reactivity Human, Rat, Mouse
Predicted Chicken, Pig, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 123799 Da
Antigen Region 12-39 aa
Additional Information
Gene ID 7414
Other Names Vinculin, Metavinculin, MV, VCL
Target/Specificity This VINC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human VINC.
Dilution WB~~1:1000
IHC-P~~1:100~500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVINC Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VCL
Function Actin filament (F-actin)-binding protein involved in cell- matrix adhesion and cell-cell adhesion. Regulates cell-surface E- cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
Cellular Location Cell membrane {ECO:0000250|UniProtKB:P12003}; Peripheral membrane protein {ECO:0000250|UniProtKB:P12003}; Cytoplasmic side {ECO:0000250|UniProtKB:P12003}. Cell junction, adherens junction {ECO:0000250|UniProtKB:P12003}. Cell junction, focal adhesion {ECO:0000250|UniProtKB:P12003}. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:P85972}. Cell membrane, sarcolemma {ECO:0000250|UniProtKB:Q64727}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q64727}; Cytoplasmic side {ECO:0000250|UniProtKB:Q64727}. Cell projection, podosome {ECO:0000250|UniProtKB:Q64727}. Note=Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions {ECO:0000250|UniProtKB:P12003}
Tissue Location Metavinculin is muscle-specific.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

VINC is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia.

REFERENCES

Moiseyeva E.P., Weller P.A.J. Biol. Chem. 268:4318-4325(1993)
Sun N., Critchley D.R., Paulin D.Biochem. J. 409:657-667(2008)
Izard T., Evans G., Borgon R.A.Nature 427:171-175(2004)

FeedBack

终于等到您。ABCEPTA(百远生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-88856768 tech-china@abcepta.com.


我要评价