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>   首页   >   产品   >   一抗   >   信号转导   >   SPRY4-Y75 Antibody   

SPRY4-Y75 Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - SPRY4-Y75 Antibody AP7446D
    All lanes: Anti-SPRY4-Y75 Antibody at 1:500 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: U-251 MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 33 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - SPRY4-Y75 Antibody AP7446D
    SPRY4-Y75 Antibody (Cat. #AP7446d) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SPRY4-Y75 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - SPRY4-Y75 Antibody AP7446D
    SPRY4-Y75 Antibody (Cat. #AP7446d) flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession Q9C004
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 32541 Da
Antigen Region 26-56 aa
Additional Information
Gene ID 81848
Other Names Protein sprouty homolog 4, Spry-4, SPRY4
Target/Specificity This SPRY4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-56 amino acids from human SPRY4.
Dilution WB~~1:500
IHC-P~~1:100~500
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSPRY4-Y75 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SPRY4
Function Suppresses the insulin receptor and EGFR-transduced MAPK signaling pathway, but does not inhibit MAPK activation by a constitutively active mutant Ras (PubMed:12027893). Probably impairs the formation of GTP-Ras (PubMed:12027893). Inhibits Ras-independent, but not Ras-dependent, activation of RAF1 (PubMed:12717443). Represses integrin-mediated cell spreading via inhibition of TESK1-mediated phosphorylation of cofilin (PubMed:15584898).
Cellular Location Cytoplasm. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note=Found in the cytoplasm in unstimulated cells but is translocated to the membrane ruffles in cells stimulated with EGF (epidermal growth factor) (By similarity). Colocalizes with TESK1 in vesicular spots in the cytoplasm (PubMed:15584898) {ECO:0000250|UniProtKB:Q9WTP2, ECO:0000269|PubMed:15584898}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

SPRY4 is an inhibitor of the receptor-transduced mitogen-activated protein kinase (MAPK) signaling pathway. The protein is positioned upstream of RAS activation and impairs the formation of active GTP-RAS.

REFERENCES

Tsai CF, et al. (2008) J Proteome Res
Guo A, et al. (2008) Proc Natl Acad Sci U S A 105, 692-7
Rikova K, et al. (2007) Cell 131, 1190-203
Wolf-Yadlin A, Hautaniemi S, Lauffenburger DA, White FM (2007) Proc Natl Acad Sci U S A 104, 5860-5
Tsumura Y, et al. (2005) Biochem J 387, 627-37

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