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Lactate Dehydrogenase A Rabbit mAb

     
  • 1 - Lactate Dehydrogenase A Rabbit mAb AP74949
    Western blot analysis of LDHA in Jurkat, rat Brain, C6, Hela lysates using Lactate Dehydrogenase A antibody.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, FC, IP
Primary Accession P00338
Reactivity Rat, Human, Mouse
Host Rabbit
Clonality Monoclonal Antibody
Isotype IgG
Conjugate Unconjugated
Purification Affinity Purified
Calculated MW 36689 Da
Additional Information
Gene ID 3939
Other Names LDHA
Dilution WB~~1:1000-1:5000
IHC-P~~1:50~200
IHC-F~~N/A
FC~~1:50-1:100
IP~~1:20-1:100
Format Liquid in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Protein Information
Name LDHA (HGNC:6535)
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Cellular Location Cytoplasm.
Tissue Location Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme.

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